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Seznam přihlášených příspěvků 2017

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Chemical Engineering II (B141b (knihovna) - 8:30)

  • Předseda: doc. Ing. Miroslav Šoóš, Ph.D.
  • Komise: Sandra Kordač Orvalho, Ph.D., Ing. Marek Šoltys, Ing. Radim Petříček, Ing. Petr Matuška (MSD)
Čas Jméno Ročník Školitel Název příspěvku Anotace
8:40 Bc. Ivana Pivarníková M2 prof. Ing. František Štěpánek, Ph.D. Synthesis of magnetic nanoparticles for remotely controlled PCR reaction detail

Synthesis of magnetic nanoparticles for remotely controlled PCR reaction

Owing to their exceptional properties, magnetic iron oxide nanoparticles (MIONs) serve promising applications in molecular biology and biomedical research. Currently, one of the objectives is to investigate the conjugation of MIONs with nucleic acid molecules (DNA/RNA). Such systems can be significant for progress in applications including detecting molecules at very low concentrations or manipulation and targeting by external magnetic field. One of the important techniques in molecular biology is the polymerase chain reaction (PCR), which allows in vitro reproduction and amplification of DNA sequences using DNA polymerase. The combination of MIONs with PCR can have beneficial but also inhibitory effect on the reaction. MIONs are widely used due to their low toxicity, physicochemical stability and ease of preparation. In presented work, dextran coated MIONs were prepared by co-precipitation method and characterized by Dynamic Light Scattering, Transmission Electron Microscopy and Fourier Transform Infrared Spectroscopy. Their heating properties were measured by Radio Frequency heating (RF). Finally, MIONs were used in PCR reaction to define the conditions for an effective PCR in the presence of MIONs and regulate the temperature cycles for PCR by RF heating. 
9:00 Bc. Jan Haša M1 prof. Ing. František Štěpánek, Ph.D. Pseudo-peptide Grafted Magnetic Nanoparticles detail

Pseudo-peptide Grafted Magnetic Nanoparticles

One of the recent challenges drug delivery systems are fronting is endosomal escape which can be prevented by co-delivery of endosomolytic pseudo-peptidic polymers. Co-delivery can be facilitated via various means such as loading the polymer directly inside a carrier or grafting the polymer on the surface of the delivery system components. Our focus turned to magnetic nanoparticles which can be employed in drug delivery systems as carriers, release triggers, contrast agent in magnetic resonance or for navigating the drug delivery system by magnetic field. In this work, a method of preparation of magnetic nanoparticles grafted with endosomolytic PP75 is described. Firstly, the surface chemistry that facilitates the grafting was studied and a standard operating protocol was optimized. The influence of the preparation method on the size distribution, colloidal stability, zeta-potential and pH responsiveness of the particles was studied.



9:20 Suada Dukaj M2 prof. Ing. František Štěpánek, Ph.D. Particle Size Effect on Release Kinetics of Drug From Suspension detail

Particle Size Effect on Release Kinetics of Drug From Suspension

Intramuscular depot formulations are considered as a way of providing constant plasma levels of a drug over extended periods of time and improving patient compliance. The principle of a depot formulation in the form of a suspension is based on the intramuscular injection of a poorly water-soluble drug, whereby the particles gradually dissolve and release the drug into systemic circulation. One of the modalities to improve the efficiency of a sustain release injectable suspensions of a poorly soluble drug into systematic circulation is to control the particle size distribution of the formulation. It is the main key parameter which controls the kinetic release of a drug delivery system. The aim of the present study is to investigate the properties of different size fractions in order to delineate how particle size governs the release profile. In order to describe the behaviour of single particle in drug dissolution, Noyes – Whitney equation was used to develop a mathematical model. This model will be used to extend the prediction of mono particle size class and later for the whole particle size distribution for understanding the mechanism and designing of drug release particles.
9:40 Maria Raquel Nunes M1 Ing. Gabriela Ruphuy, Ph.D. Yeast-derived beta glucan particles for the encapsulation of low-water soluble payloads detail

Yeast-derived beta glucan particles for the encapsulation of low-water soluble payloads

Yeast-derived glucan particles (GPs) are biopolymeric microspheres consisting of the cell wall of Saccharomyces cerevisiae (baker’s yeast). They are obtained after the removal of yeast internal organelles, resulting in hollow and porous particles that are mainly composed of β-glucan. These particles exhibit immunomodulatory activity, which is the main reason for its use in different applications, such as the production of functional foods and targeted drug delivery. Previous investigations regarding the encapsulation in glucan particles has been focused mainly in water-soluble payloads, such as DNA, liposomes, siRNA peptides and small molecules. However, the encapsulation of low water-soluble payloads, such as curcumin, ibuprofen and other active pharmaceutical ingredients (APIs) is still an area to explore, being the focus of this work. Spray drying technique was used for the formulation of the β-glucan particles loaded with the low-water soluble payloads, where different parameters and different API/GP mass ratios were used, in order to analyze their influence in encapsulation efficiency and dissolution kinetics. Curcumin was used as low-water soluble model drug, thus, pure curcumin and curcumin/GP composites were produced and characterized by UV-Vis spectrophotometry.
10:20 Vladimír Němec B3 prof. Ing. František Štěpánek, Ph.D. Effect of granulation process parameters on the dissolution of IBU-lactose formulations detail

Effect of granulation process parameters on the dissolution of IBU-lactose formulations

The aim of the study is to create a method, which allows the processing of a powder mixture of ibuprofen and lactose by high shear granulation in a laboratory scale granulator. Mapping of the parametric space and optimization of the granulation process are two crucial points, thus various experimental set-ups will be examined (i.e. batch volumes and different impeller speed). Granules will be prepared from different size fractions of ibuprofen and lactose. Powders for granulation will be prepared by sieving and the chosen fractions will be used for the size effect study. The produced granulate will be characterized by measuring particle size distribution, liquid to solid ratio necessary for achieving a properly granulated batch, and granule strength. The granulated powders will serve as a material for a further study of granule microstructure, content uniformity, dissolution and tabletting.
10:40 David Zůza B3 Ing. Tokárová Viola, PhD. Design of micro-reaction apparatus for continuous synthesis of silica particles detail

Design of micro-reaction apparatus for continuous synthesis of silica particles

Silica particles are subject of intense research in the last 20 years especially, because they are thermally and chemically stable and can be prepared in various shapes and sizes with a porous structure. One of the possible utilizations is their use as drug carriers for an oral drug delivery where some variants are FDA approved. However, usual batch preparation methods of silica with high porosities usually lead to polydispersity of final particles. Transferring the synthesis into a flow-through device with higher control over hydrodynamic conditions could lower the polydispersity and provide a continuous production process. This can be done using a CSTR, a plug-flow reactor or even a microfluidic chip. However, it is necessary to take into account a wide spectrum of parameters that needs to be set, such as reaction stoichiometry, reaction time and hydrodynamic conditions. In this work, a system for continuous preparation of silica microparticles with high porosity (800 m2/g) is designed and tested. After considering all aspects described above, several reactor designs are proposed and 3D printed from ABS. Synthetized silica microparticle in purposefully-designed reactors are characterized by optical microscopy, scanning electron microscopy and static light scattering.



11:00 Bc. Sarah Akhlasová M1 prof. Ing. František Štěpánek, Ph.D. Retardation of API dissolution by formulation approaches detail

Retardation of API dissolution by formulation approaches

The goal of generic pharmaceutical development is a production of dosage form bioequivalent with original drug product. Mostly, alternative solid form (polymorph or amorphous form) of active pharmaceutical ingredient (API) is used, due to the patent protection of the original. Various solid forms differ in physical - chemical properties, e.g. in dissolution behavior of API, which is one of the most important properties of the drug product. Fast API dissolution can lead to the failure of the developed prototype in bioequivalence study and moreover endanger patient safety. Thus, there is a need to retard the dissolution rate of API, if chosen API solid form has faster dissolution than is required. In this work, the retardation is done by appropriate formulation of the drug product. Polymers with promising properties for retardation were chosen and simulation of wet granulation with API in small scale, followed by the dissolution of granulates to study polymers capability of retardation was performed. Later, various technologies of manufacturing of drug product – direct compression, roller compaction, high–shear and fluid bed granulation were studied to determine the impact of the technology on the retardation. So far, some of the polymers showed the retardation of API dissolution.
11:20 Bc. Jakub Mužík M2 prof. Ing. František Štěpánek, Ph.D. In situ drug amorphisation by microwave irradiation stabilized by mesoporous silica detail

In situ drug amorphisation by microwave irradiation stabilized by mesoporous silica

A common issue in the pharmaceutical industry is low solubility and dissolution rate of Active Pharmaceutical Ingredients (APIs). The dissolution rate might be significantly enhanced by changing state of the matter from crystalline to amorphous one. Carriers or stabiliser such as polymer or porous materials, which can absorb or lock the molecules of API in its structure, are used to keep amorphous state of the API. Powders composed from ibuprofen as model API and mesoporous silica micro-particles as a carrier were experimentally investigated for in situ amorphization of ibuprofen by microwave (MW) radiation. As a source of microwaves commercially available MW oven was used and characterized. The MW power input and composition of the tablets (excipients/ibuprofen) were tested with respect to the melting point of ibuprofen (78°C). The changes of the crystalline structure before and after MW treatment were analysed by X-Ray Diffractometry (XRD) and Differential Scanning Calorimetry (DSC). Dissolution profiles of samples were evaluated by HPLC. Scanning electron and optical microscopy were used for the particle structure characterization. For further scale-up fabrication a fluid amorphiztor was constructed and experimentally tested.
11:40 Bc. Vojtěch Klimša M2 Ing. Ondřej Kašpar, Ph.D. Continuous spray drying with variable inlet formulation - design and optimization detail

Continuous spray drying with variable inlet formulation - design and optimization

Spray drying is one of the most known unit operations for the production of powder materials and because of short contact times also one of least likely to damage heat-sensitive components. Therefore, it is widely used in pharmaceutical and food industry. The focus of this study is to design and optimize a system that converts conventional batch-wise spray drying operation into continuous one with the minimal risk of product cross-contamination. The proposed set-up allows time-efficient screening of products with various composition. For this task, a custom designed valve was developed along with a procedure allowing effective cleaning of separation cyclone during the run. Finally, the experiment was carried out to demonstrate the feasibility of modification and degree of cross-contamination across all sample fractions.
Aktualizováno: 10.10.2017 12:27, Autor: Martin Mastný

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