čas: 23.4.2021 00:21:22
Obnovit | RAW
Food Research Institute Prague
List of available PhD theses
Molecular characterization of various variants of fish parvalbumin gene in respect of biochemical and allergenic features of corresponding expressed proteins
Fish parvalbumin gene is composed of four exons separated by three introns. The gene encodes highly thermoresistant parvalbumin protein. This protein can act as food allergen in predisposed consumers. Intron sequences of the gene can due to their uniqueness serve as species determination marker, while exons encode protein, including respective epitopes triggering allergic reaction. In each fish species, parvalbumin gene is represented by number of paralogs. The aim of the dissertation is to characterize these paralog/ortholog variants by means of molecular tools as well as by biochemical and immunochemical approaches, building on phylogenetic data obtained from databases as well as from NGS. Obtained dataset should also serve as a base for next construction of microarray chip for species determination or in phagemid version for pacient sera profiling to assess reactivity to a spectrum of various fish species meat.